Anti-CCD absorbent

Clinical Information

Anti-CCD absorbent is an additional reagent for the incubation with blot-based allergy profiles. The absorbent eliminates anti-CCD IgE antibodies from the patient serum or plasma, which increases the specificity of the test results if these antibodies are present in the sample.

Anti-CCD IgE antibodies are directed against sugar structures of proteins and can be detected in around 25% of allergy patients as well as in non-allergic individuals. They generally have no clinical relevance.

In extract-based in vitro allergy diagnostics, these antibodies complicate the interpretation of positive results as the following cases cannot be differentiated:

• positive reaction caused by IgE antibodies against peptide epitopes
• positive reaction caused by anti-CCD IgE antibodies
• positive reaction caused by a combination of the two antibody types

Differentiation between the described reactions is possible when the absorbent is used and the anti-CCD IgE antibodies are eliminated.

The anti-CCD absorbent is useful if the patient serum or plasma is shown to contain IgE antibodies against CCD structures. This is indicated by a positive CCD band on the incubated allergy profile. In this case, the serum or plasma should be retested using the anti-CCD absorbent.

Diagnostics

The patient sample is incubated with anti-CCD absorbent for 60 minutes at room temperature according to the instructions. The sample can then be used directly for incubation with the respective allergy profile.